Daten 2013 en

Data of the NRZMHi for Haemophilus influenzae 2013

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1. Introduction

 

The Consulting Laboratory for Haemophilus influenzae (KLHi) has been merged with the National Reference Laboratory for Meningococci in 2013. Both laboratories henceforth will be called National Reference Laboratory for Meningococci and Haemophilus influenzae (NRZMHi). The tasks assigned by the Robert Koch Institute for the surveillance of invasive Haemophilus influenzae disease include serotyping of clinical isolates and the detection of antibiotic resistance against β-Lactams.In 2013, 349 submissions from 344 patients were analysed. In 288 of the submitted cases, H. influenzae was detected from primarily sterile materials (this figure equals the number of invasive diseases); in two submissions from patients with invasive disease, H. haemolyticus was identified. The differentiation of both species may be challenging. At the NRZMHi, it is accomplished in doubtful cases by molecular biologic analysis of the genes ompP2, ompP6 and fucK.The age group most affected by invasive H. influenzae disease was > 40 years (247 cases, 86.1 %). The vast majority of invasive cases was caused by non-typeable H. influenzae (NTHi, 250 cases, 87.1 %), followed by Hif as the most frequent capsular serotype (22 cases; 7.7 %). Hie showed third highest frequency among the serotypes (10 cases; 3.5 %), closely followed by Hib (3 cases, 1 %) and Hia (2 cases, 1 %). In one case, where detection of the bacteria was carried out by molecular methods, the serotype was not determined.The NRZMHi analyzed the frequency of ampicillin resistance of 287 viable invasive isolates using Etests. Fourty-two isolates (14.7 %) were ampicillin resistant (MIC > 1 µg/ml), of which 32 isolates (71.2 %) showed β lactamase production.

 

4. Serotype distribution in Federal States

 

	BW	BY	BE	BB	HB	HH	HE	MV	NI	NW	RP	SL	SN	SA	SH	TH
Hia	1	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0
Hib	1	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0
Hie	3	1	1	0	0	0	0	0	1	2	2	0	0	0	0	0
Hif	4	5	1	0	0	0	4	0	1	4	1	0	0	1	0	1
NTHi	52	47	14	7	0	4	18	3	13	54	11	6	7	4	7	3
n.d.	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0
Total	61	53	16	7	0	4	22	3	16	60	15	6	9	5	7	4

BW: Baden-Württemberg, BY: Bavaria, BE: Berlin, BB: Brandenburg, HB: Bremen, HH: Hamburg, HE: Hesse, MV: Mecklenburg-Vorpommern, NI: Lower Saxony, NW: North Rhine-Westfalia, RP: Rhineland-Palatinate, SL: Saarland, SN: Saxony, ST: Saxony-Anhalt, SH: Schleswig-Holstein, TH: Thuringia.

5. Serotype distribution according to site of isolation

	Hia	Hib	Hie	Hif	NTHi	n.d.	Total
Blood	1	3	8	22	232	1	267
CSF	1	0	1	0	15	0	17
other*	0	0	1	0	3	0	4

* other – primarily sterile sites: pleural effusion

 

7. Publications of the KLHi

Original Articles

- Lâm TT, Elias J, Frosch M, Vogel U, Claus H. New diagnostic PCR for Haemophilus influenzae serotype e based on the cap locus of strain ATCC 8142. Int J Med Microbiol. 2011 Feb;301(2):176-9.

- Lâm TT, Frosch M, Claus H, Vogel U. Sequence analysis of the serotype-specific synthesis regions II of Haemophilus influenzae serotypes c and d: evidence for common ancestry of capsule synthesis in Pasteurellaceae and Neisseria meningitidis. Research in Microbiology 2011 Jun;162(5):483-7.

Reviews

- Lâm TT, Claus H, Elias J, Hellenbrand W, Imöhl M, Prelog M, Sing A, van der Linden M, Vogel U. Infections with Pneumococci, Menigococci, H. influenzae and Diphtheria in Germany: the RKI Reference Network for Invasive Bacterial Infections (IBI) at the 5th Würzburg Workshop on Epidemiology, Prevention and Therapy for Invasive Meningococcal Diseases 2010 (Meeting Report). Gesundheitswesen. 2011. DOI: 10.1055/s-0031-1286269.

Disclaimer: the above data were generated with federal funds (RKI). Scientific use is prohibited without prior written consent by KLHi or RKI. Commercial use is strictly prohibited. Inclusion of figures or tables in talks or oral presentations is not allowed.