Gene Expression analyses
To further improve our understanding of Neisseria-host cell interactions, we have analysed host cell gene expression profiles in infected HBMEC using cDNA microarrays. Recent advances in genomic, including human sequencing, pathogen sequencing and high-throughput gene expression profiling techniques offered new opportunities for studying the molecular cellular responses to defined perturbations. In particular, global gene expression analysis is a powerful tool for studying the interaction between host cells and microbial pathogens or their products that occur at the host-microbe interface
including that at the blood-brain barrier. The results of our studies indicated that meningococci regulate transcription of a wide range of genes involved in transcription, translation and the cell metabolism and genes encoding for intracellular transducers. In addition, we identified several differentially regulated genes not previously known to respond to N. meningitidis infection.
Joseph, B., Frosch, M., Schoen, C. and Schubert-Unkmeir, A. Transcriptome analyses in the interaction of Neisseria meningitidis with mammalian host cells. Methods Mol. Biol. , 799:267-93, 2012.
Schubert-Unkmeir A, Slanina H, Frosch M. Mammalian cell transcriptome in response to meningitis-causing pathogens. Expert Rev Mol Diagn. 9(8):833-42, 2009.
Schubert-Unkmeir A, Schramm-Glück A, Frosch M, Schoen C. Transcriptome analyses in the interaction of Neisseria meningitidis with mammalian host cells. Methods Mol. Biol. 470:5-27, 2009.
Schubert-Unkmeir, A., Sokolova, O., Panzner, U., Eigenthaler, M. and Frosch, M. Gene expression pattern in human brain endothelial cells in response to Neisseria meningitidis. Infect. Immun. 75:899-914, 2007.