Deutsch Intern
    Institut für Hygiene und Mikrobiologie

    New Paper in Front. Cell Dev. Biol.


    In a current collaboration project with the group of Prof. M Sauer (http://www.super-resolution.biozentrum.uni-wuerzburg.de/startseite/) Jan Schlegel (AG Markus Sauer) and Simon Peters (AG Schubert-Unkmeir) investigated the mobility of CD147 upon N. meningitidis infection by single-molecule tracking experiments. They analysed the role of the two sphingolipids GM1 and Gb3 during infection with N. meningitidis using fluorescently labeled CTxB and STxB subunits, respectively. Super-resolution microscopy by structured illumination microscopy (SIM) and direct stochastic optical reconstruction microscopy (dSTORM) showed GM1 accumulation around meningococci highlighting their significant importance for bacterial invasion.


    Schlegel J, Peters S, Doose S, Schubert-Unkmeir A, Sauer M (2019) Super-resolution microscopy reveals local accumulation of plasma membrane gangliosides at Neisseria meningitidis invasion sites. Front Cell Dev Biol. 2019 Sept 7, 194.