Scintific Reports nature publishing group05.03.2021
Click-correlative light and electron microscopy for imaging and tracking azido-functionalized sphingolipids in bacteria
In a collaboration project between researchers from IHM (Simon Peters and Lena Kaiser, AG Schubert-Unkmeir) together with the groups of Prof. J. Seibel, Prof. M. Sauer, Prof. C. Stigloher and Prof. B. Kleuser, introduce ‘click-AT-CLEM’, a labeling technique for correlated light and electron microscopy (CLEM) based on the super-resolution array tomography (srAT) approach and bio-orthogonal click chemistry for imaging of azido-tagged sphingolipids to directly visualize their interaction with the model Gram-negative bacterium Neisseria meningitidis at subcellular level. Click-AT-CLEM imaging and mass spectrometry clearly revealed efficient incorporation of azido-tagged sphingolipids into the outer membrane of Gram-negative bacteria as underlying cause of their antimicrobial activity.
Peters, S., Kaiser, L., Fink, J. Schumacher, F., Perschin, V., Schlegel, J. , Sauer, M., Stigloher, C., Kleuser, B., Seibel, J. and Schubert-Unkmeir, A. Click-correlative light and electron microscopy (click-AT-CLEM) for imaging and tracking azido-functionalized sphingolipids in bacteria. Sci Rep 11, 4300 (2021). https://doi.org/10.1038/s41598-021-83813-w
Funding FOR2123/DFG and GRK2581/DFG