Intern
    Institut für Hygiene und Mikrobiologie

    Publications

     

    2017

    Burgert A, Schlegel J, Bécam J, Doose S, Bieberich E, Schubert-Unkmeir A, Sauer M.Characterization of Plasma Membrane Ceramides by Super-Resolution Microscopy. Angew Chem Int Ed Engl. 2017 Apr 5. doi: 10.1002/anie.201700570.

    Dick J, Hebling S, Becam J, Taha MK, Schubert-Unkmeir A. Comparison of the Inflammatory Response of Brain Microvascular and Peripheral Endothelial Cells following Infection with Neisseria meningitidis. Pathog Dis. 2017 Apr 1. doi: 10.1093/femspd/ftx038.

    Schubert-Unkmeir A. Molecular mechanisms involved in the interaction of Neisseria meningitidis with cells of the human blood-cerebrospinal fluid barrier. Pathog Dis. 2017 Mar 1;75(2). doi: 10.1093/femspd/ftx023.

    2016

    Simonis A, Schubert-Unkmeir A. Interactions of meningococcal virulence factors with endothelial cells at the human blood-cerebrospinal fluid barrier and their role in pathogenicity. FEBS Lett. 2016 Aug 6. doi: 10.1002/1873-3468.12344.

    von Papen M, Oosthuysen WF, Becam J, Claus H, Schubert-Unkmeir A.
    Disease and Carrier Isolates of Neisseria meningitidis Cause G1 Cell Cycle Arrest in Human Epithelial Cells. Infect Immun. 2016 Sep 19;84(10):2758-70.

    Walter T, Collenburg L, Japtok L, Kleuser B, Schneider-Schaulies S, Müller N, Becam J, Schubert-Unkmeir A, Kong JN, Bieberich E, Seibel J.
    Incorporation and visualization of azido-functionalized N-oleoyl serinol in Jurkat cells, mouse brain astrocytes, 3T3 fibroblasts and human brain microvascular endothelial cells. Chem Commun (Camb). 2016 Jun 30;52(55):8612-4.

    Doran KS, Fulde M, Gratz N, Kim BJ, Nau R, Prasadarao N, Schubert-Unkmeir A, Tuomanen EI, Valentin-Weigand P. Host-pathogen interactions in bacterial meningitis. Acta Neuropathol. 2016 Feb;131(2):185-209.

    Oosthuysen WF, Mueller T, Dittrich MT, Schubert-Unkmeir A. Neisseria meningitidis causes cell cycle arrest of human brain microvascular endothelial cells at S phase via p21 and cyclin G2. Cell Microbiol. 2016 Jan;18(1):46-65.


    2015

    Oosthuysen, W. F., Mueller, T., Dittrich, M. T. and Schubert-Unkmeir, A. Neisseria meningitidis causes cell cycle arrest of human brain microvascular endothelial cells at S- phase via p21 and cyclin G2. Cell Microbiol. 2015 Jul 7. doi: 10.1111/cmi.12482.

    Schubert-Unkmeir, A. and Doran, K.S.  Mechanisms of bacterial interaction with cells of the blood-cerebrospinal fluid barrier. In ‘Vascular Responses to pathogens’. Gavins F  and Stokes K eds. Academic Press Press INC, 2015, ISBN 9780128010785

    2014

    Simonis, A., Hebling, S., Gulbins, E., Schneider-Schaulies, S. and Schubert-Unkmeir, A. Differential activation of acid sphingomyelinase and ceramide release determines invasiveness of Neisseria meningitidis into brain endothelial cells. PLoS Pathog. 2014 Jun 12;10(6):e1004160.

    Slanina H, Mündlein S, Hebling S, Schubert-Unkmeir A. Role of epidermal growth factor receptor signaling in the interaction of Neisseria meningitidis with endothelial cells. Infect Immun. Infect Immun. 2014 Mar;82(3):1243-55.

    2013

    Slanina H, Mündlein S, Hebling S, Schubert-Unkmeir A. Role of epidermal growth factor receptor signaling in the interaction of Neisseria meningitidis with endothelial cells. Infect Immun. 2013 Dec 30. [Epub ahead of print]

    Schubert-Unkmeir A, and Christodoulides M. Genome-based bacterial vaccines: current state and future outlook. BioDrugs. 2013 Oct; 27(5):419-30.

    2012

    Slanina H, Schmutzler M, Christodoulides M, Kim KS, Schubert-Unkmeir A. Effective plasmid DNA and small interfering RNA delivery to diseased human brain microvascular endothelial cells. J Mol Microbiol Biotechnol. 2012;22(4):245-57.

    Slanina H, Hebling S, Hauck CR, Schubert-Unkmeir A. Cell Invasion by Neisseria meningitidis Requires a Functional Interplay between the Focal Adhesion Kinase, Src and Cortactin. PLoS One. 7:e39613. 2012.

    Müller I, Freitag MH, Poggensee G, Scharnetzky E, Straube E, Schoerner Ch, Hlobil H, Hagedorn HJ, Stanek G, Schubert-Unkmeir A, Norris DE, Gensichen J, Hunfeld KP. Evaluating frequency, diagnostic quality, and cost of Lyme borreliosis testing in Germany: a retrospective model analysis. Clin Dev Immunol. 2012:595427, 2012.

    Joseph B, Frosch M, Schoen C, Schubert-Unkmeir A. Transcriptome analyses in the interaction of Neisseria meningitidis with mammalian host cells. Methods Mol Biol. 799:267-93, 2012.

    2011

    Kobsar, A., Siauw, C., Gambarayan, S., Speer, C., Schubert-Unkmeir, A.*, and Eigenthaler, M. *; *contributed equally. Neisseria meningitidis induces platelet inhibition and increases vascular endothelial permeability via nitric oxide regulated pathways. Thromb Haemost. 106:1127-38, 2011.

    Schielke S, Spatz C, Schwarz RF, Joseph B, Schoen C, Schulz SM, Hubert K, Frosch M, Schubert-Unkmeir A, Kurzai O. Characterization of FarR as a highly specialized, growth phase-dependent transcriptional regulator in Neisseria meningitidis. Int J Med Microbiol. 2011 Apr;301(4):325-33.

    Slanina H, König A, Claus H, Frosch M, Schubert-Unkmeir A. Real-time impedance analysis of host cell response to meningococcal infection.
    J Microbiol Methods. Jan;84(1):101-8., 2011  Epub 2010 Nov 13.

     

    2010

     

    Schubert-Unkmeir, A., Konrad, C., Slanina, H., Czapek, F., Hebling, S., and Frosch, M. Neisseria meningitidis induces endothelial cell detachment from the matrix and cleavage of occludin: a role for MMP-8 activity. PLoS Pathog. Apr 29;6(4):e1000874, 2010

    Schielke S, Schmitt C, Spatz C, Frosch M, Schubert-Unkmeir A, Kurzai O. The transcriptional repressor FarR is not involved in meningococcal fatty acid resistance mediated by the FarAB efflux pump and dependent on LPS structure. Appl Environ Microbiol. Mar 26. [Epub ahead of print], 2010.

    Slanina H, König A, Hebling S, Hauck CR, Frosch M, Schubert-Unkmeir A. Entry of N. meningitidis into mammalian cells requires Src family Protein-tyrosine kinases. Infect Immun. May;78(5):1905-14 , 2010.

     

    2009

     

    Schubert-Unkmeir A, Slanina H, Frosch M. Mammalian cell transcriptome in response to meningitis-causing pathogens. Expert Rev Mol Diagn. 9(8):833-42, 2009.

    Fischer, C.B., Ehrenhaft, G., Schubert-Unkmeir, A. and Wehner, S. The interaction of bacteria with single wall carbon nanotube (SWCNT) films: A SEM study. The Open Surface Science Journal, 1:50-53, 2009

    Schielke, S., Huebner, Spatz, C., Nägele, V., Ackermann, N., Frosch, M., Kurzai, O., and Schubert-Unkmeir, A. Expression of the meningococcal adhesion NadA is controlled by a transcriptional regulator of the MarR-familiy Mol. Microbiol., 72:1054-1067, 2009. (* listed by the faculty 1000 Biology factor 6 )

    Schubert-Unkmeir A, Schramm-Glück A, Frosch M, Schoen C. Transcriptome analyses in the interaction of Neisseria meningitidis with mammalian host cells. Methods Mol. Biol. 470:5-27, 2009.

     

    Vor 2009


    Schubert-Unkmeir, A., Sokolova, O., Panzner, U., Eigenthaler, M. and Frosch, M. Gene expression pattern in human brain endothelial cells in response to Neisseria meningitidis. Infect. Immun. 75:899-914, 2007.

    Nikulin, J., Panzner, U., Frosch, M. and Schubert-Unkmeir, A. (2006) Intracellular survival and replication of Neisseria meningitidis in human brain microvascular endothelial cells Int J Med Microbiol. 296: 553-558.

    Siauw, C., Kobsar, A., Dornieden C, Beyrich C, Schinke B, Schubert-Unkmeir, A., Abele-Horn, M., Speer, C., Eigenthaler, M. (2006) Group B streptococcus isolates from septic patients and healthy carriers differentially activate platelet signalling cascades. Thromb Haemost. 95: 836-849.

    Sokolova, O., Heppel, N., Jägerhuber, R., Kim, K.S., Frosch, M., Eigenthaler M. and Schubert-Unkmeir, A. (2004) Interaction of Neisseria meningitidis with human brain microvascular endothelial cells: role of MAP- and tyrosine kinases in invasion and inflammatory cytokine release. Cell Microbiol. 6: 1153-1166.

    Unkmeir, A., Latsch, K., Dietrich, G., Wintermeyer, E., Schinke, B., Schwender, S., Kim, K.S., Eigenthaler, M, and Frosch, M. (2002)  Fibronectin mediates Opc-dependent internalization of Neisseria meningitidis in human brain microvascular endothelial cells. Mol Microbiol. 46: 933-946.

    Guckenberger, M., S. Kurz, C. Aepinus, S. Theiss, S. Haller, T. Leimbach, U. Panzner, J. Weber, H. Paul, A. Unkmeir, M. Frosch, and G. Dietrich. (2002) Analysis of the Heat shock Response of Neisseria meningitidis with cDNA and Oligonucleotide-Based DNA microarrays. J Bacteriol. 184: 2546-2551.

    Unkmeir, A., U. Kämmerer, A. Stade, C. Hübner, S. Haller, A. Kolb-Mäurer, M. Frosch, and G. Dietrich. (2002)  Lipooligosaccharide and polysaccharide capsule: virulence factors of Neisseria meningitidis that determine meningicoccal interaction with human dendritic cells. Infect Immun. 70: 2454-2462.

    Kolb-Mäurer, A., A. Unkmeir, U. Kämmerer, C. Hübner, T. Leimbach, A. Stade, E. Kämpgen, M. Frosch and G. Dietrich. (2001)  Interaction of Neisseria meningitidis with Dendritic Cells. Infect Immun. 69: 6912-6922.

    Unkmeir, A. and Schmidt, H. (2000) Structural analysis of phage-borne stx genes and their flanking sequences in shiga toxin-producing Escherichia coli and Shigella dysenteriae type 1 strains. Infect Immun. 68: 4856-4864.

    Hammerschmidt, S., Hilse, R., van Putten, J.P., Gerardy-Schahn, R., Unkmeir, A. and Frosch, M. (1996) Modulation of cell surface sialic acid expression in Neisseria meningitidis via a transposable genetic element. Embo J. 15: 192-198.


    Reviews and Book Chapters

    Schubert-Unkmeir A, and Christodoulides M. Genome-based bacterial vaccines: current state and future outlook. BioDrugs. 2013 Oct; 27(5):419-30.

    Schubert-Unkmeir A, Slanina H, Frosch M. Mammalian cell transcriptome in response to meningitis-causing pathogens.
    Expert Rev Mol Diagn. 2009 Nov;9(8):833-42.

    Schubert-Unkmeir A., A. Schramm-Glück, M. Frosch and C. Schoen Transcriptome analyses in the interaction of Neisseria meningitidis with mammalian host cells.  Methods Mol. Biol. 470:5-27, 2009

    Drittmittelförderung/Research Funding:

    aktuell

    Deutsche Forschungsgemeinschaft

    „Untersuchung zur funktionellen Relevanz von Sphingomyelinasen und Ceramiden in der Pathogenese der Meningokokkeninfektion“ (SCHU 2394/2-1)
    Teilprojekt P03 im Rahmen der Forschergruppe FOR 2123 „Sphingolipids in infection control“
    Laufzeit: 01/2014-12/2016

    BaCaTec (Bavaria California Technology Center)
    “Role of non-receptor tyrosine kinases and activated signaling pathways in bacterial adherence and invasion of host cells” (Förederprojekt Nr. 12 [2013-2])
    Prof. A. Schubert-Unkmeir and Prof. Kelly S. Doran, San Diego State University
    Laufzeit: 01/2014-12/2015

    Untersuchungen zur Integrin-vermittelten Signalweiterleitung in Endothelzellen nach Infektion mit N. meningitidis – Mechanismen zur Überwindung der Blut-Hirn-Schranke (SCHU-2394/1-2
    Fortsetzungsantrag (SCHU-2394/1-2)
    Laufzeit:  10/2010 – 10/2013

    „Untersuchungen zur Integrin-vermittelten Signalweiterleitung in Endothelzellen nach Infektion mit N. meningitidis“
    Normalverfahrensantrag  (SCHU-2394/1-1)
    Laufzeit:  10/2007 – 10/2010

    „Interaction of Neisseria meningitidis with cells of the human blood brain barrier“
    Sonderschwerpunktprogramm SPP1130 “Infektionen des Endothels”
    Dr. A. Schubert-Unkmeir, PD Dr. M. Eigenthaler

    Interdisziplinäres Zentrum für Klinische Forschung der Universität Würzburg (IZKF):
    Pathologische Aktivierung humaner Thrombozyten durch Meningokokken und Gruppe B Streptokokken: Molekulare Mechanismen und klinische Bedeutung
    PD Dr. M. Eigenthaler, Dr. A. Schubert-Unkmeir, Prof. Dr. Ch. P. Speer

    Kontakt

    Institut für Hygiene und Mikrobiologie
    Josef-Schneider-Straße 2
    97080 Würzburg

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