Intern
    Institut für Hygiene und Mikrobiologie

    Publications

    Rudelius, M., Brehm, K., Poelcher, M., Spinner, C., Rosenwald, A., Prazeres da Costa, C. (2017) First case of human peritoneal cysticercosis mimicking peritoneal carcinosis: necessity of laparoscopy and histologic assessment for the correct diagnosis. JMM Case Reports, in press.

    Ancarola, M.E., Marcilla, A., Herz, M., Macchiaroli, N., Pérez, M., Asurmendi, S., Brehm, K., Poncini, C., Rosenzvit, M., Cucher, M. (2017) Cestode parasites release extracellular vesicles with microRNAs and antigenic protein cargo. Int. J. Parasitol., in press.

    Monteiro, K.M., Lorenzatto, K.R., Camargo, J., dos Santos, G.B., Lorenz, S., Paludo, G.P., Carvalho, P.C., Zaha, A., Brehm, K., Ferreira, H.B. (2017). Comparative proteomics of hydatid fluids from Echinococcus multilocularis and Echinococcus granulosus cestode parasites. J. Proteomics, in press.

    Brehm, K. (2017) Die Echinokokkose – Eine Übersicht und neue Erkenntnisse in der Diagnostik, Therapie und Parasitenbiologie. Epidemiol. Bull. 15: 127-132.

    Brehm, K., Koziol, U. (2017) Echinococcus – Host interactions at cellular and molecular levels. Advances in Parasitology 95: 147-212.

    Vendelova E, Camargo de Lima J, Lorenzatto KR, Monteiro KM, Mueller T, Veepaschit J, Grimm C, Brehm K, Hrčková G, Lutz MB, Ferreira HB, Nono JK. (2016) Proteomic Analysis of Excretory-Secretory Products of Mesocestoides corti Metacestodes Reveals Potential Suppressors of Dendritic Cell Functions. PLoS Negl Trop Dis. 10:e0005061.

    Vendelova E, Hrčková G, Lutz MB, Brehm K, Nono Komguep J. (2016) In vitro culture of Mesocestoides corti metacestodes and isolation of immunomodulatory excretory-secretory products. Parasite Immunol. 38:403-13.

    Koziol, U., Koziol, M., Preza, M., Costábile, A., Brehm, K., Castillo, E. De novo discovery of neuropeptides in the genomes of parasitic flatworms using a novel comparative approach. International Journal for Parasitology 46: 709-721.

    Koziol, U., Jarero, F., Olson, P.D., Brehm, K. (2016) Comparative analysis of Wnt expression identifies a highly conserved developmental transition in flatworms. BMC Biology 14:10.

    del Puerto, L., Rovetta, R., Navatta, M., Fontana, E.C., Lin, G., Moyna, G., Dematteis, S., Brehm, K., Koziol, U., Ferreira, F., Diaz, A. Negligible elongation of mucin glycans with Gal β1-3 units distinguishes the laminated layer of Echinococcus multilocularis from that of E. granulosus. Int J Parasitol 46: 311-321.

    Jurke, A., Bannert, N., Brehm, K., Fingerle, V., Kempf, V.A., Kömpf, D., Lunemann, M., Mayer-Scholl, A., Niedrig, M., Nöckler, K., Scholz, H., Splettstoesser, W., Tappe, D., Fischer, S,F. (2015) Serological survey of Bartonella spp., Borrelia burgdorferi, Brucella spp., Coxiella burnetii, Francisella tularensis, Leptospira spp., Echinococcus, Hanta-, TBE- and XMR-virus infection in employees of two forestry enterprises in North Rhine-Westphalia, Germany, 2011-2013. Int J Med Microbiol. 305: 652-662.

    Koziol, U., Radio, S., Smircich, P., Zarowiecki, M., Fernández, C., Brehm, K. (2015) A novel terminal-repeat retrotransposon in miniature (TRIM) is massively expressed in Echinococcus multilocularis stem cells. Genome Biology and Evolution 7: 2136-2153.

    Monoranu, C.M., Müllges, W., Keppler, M., Brehm, K., Ondrejka, S.L., Muntau, B., Tannich, E., Müller-Hermelink, H.K., Tappe, D. (2015) Molecular and histopathological diagnosis of fatal meningoencephalitis due to Halicephalobus nematodes in human, Germany, 2014. Open Forum Infectious Diseases 2: ovf061.

    Koziol, U., Brehm, K. (2015) Recent advances in Echinococcus genomics and stem cell research. Vet. Parasitol. 213: 92-102.

    Cucher, M., Macchiaroli, N., Kamenetzky, L., Maldonado, L., Brehm, K., Rosenzvit, M. (2015) High throughput characterization of Echinococcus spp. metacestodes miRNomes. Int. J. Parasitol. 45: 253-267.

    Brehm, K., Koziol, U. (2014) On the importance of targeting parasite stem cells in anti-echinococcosis drug development. Parasité 21: 72.

    Schubert, A., Koziol, U., Cailliau, K., Vanderstraete, M., Dissous, C., Brehm, K. (2014) Targeting Echinococcus multilocularis stem cells by inhibition of the Polo-like kinase EmPlk1. PLoS Negl. Trop. Dis. 6: e2870.

    Skinner, D.E., Rinaldi, G., Koziol, U., Brehm, K., Brindley, P.J. (2014) How might flukes and tapeworms maintain genome integrity without a canonical piRNA pathway? Trends Parasitol. 30: 123-129.

    Koziol, U., Rauschendorfer, T., Zanon-Rodriguez, L., Krohne, G. and Brehm, K. (2014) The unique stem cell system of the immortal larva of the human parasite Echinococcus multilocularis. EvoDevo 5: 10.

    Nono, J.K., Lutz, M.B., and Brehm, K. (2014) EmTIP, a T-cell immunomodulatory protein secreted by the tapeworm Echinococcus multilocularis is important for early metacestode development. PLoS Negl. Trop. Dis. 8: e2632.

    Hemer, S., Konrad, C., Spiliotis, Koziol, U., M., Schaack, D., Förster, S., Gelmedin, V., Stadelmann, B., Dandekar, T., Hemphill, A., Brehm, K.* (2014) Host insulin stimulates Echinococcus multilocularis insulin signalling pathways and larval development. BMC Biol. 12: 5.

    Hsu, T.L., Lin, G., Koizumi, A., Brehm, K., Hada, N., Chuang, P.K., Wong, C.H., Hsieh, S.L., and Diaz, A. (2013) The surface carbohydrates of the Echinococcus granulosus larva interact selectively with the rodent Kupffer cell receptor. Mol. Biochem. Parasitol. 192: 55-59.

    Brehm, K. (2013)
    Protein kinases as drug targets in the treatment of alveolar echinococcosis. In Protein phosphorylation in eukaryotic parasites: novel targets for antiparasitic intervention. Drug discovery in infectious diseases (vol. 5). Edited by Doerig, C., Wiese, M., Spaeth, G. Wiley-VCH 2013; in press.

    Nono, J.K., Lutz, M.B., and Brehm, K. EmTIP, a T-cell immunomodulatory protein secreted by the tapeworm Echinococcus multilocularis is important for early metacestode development. PLoS Negl. Trop. Dis., in press.

    Koziol, U., Krohne, G., Brehm, K. (2013) Anatomy and development of the larval nervous system in Echinococcus multilocularis. Front. Zool. 10: 24.

    Camicia, F., Herz, M., Prada, L.C., Kamenetzky, L., Simonetta, S.H., Cucher, M.H., Bianchi, J.I., Fernandez, C., Brehm, K., Rosenzvit, M.C. (2013) The nervous and prenervous roles of serotonin in parasitic flatworms Echinococcus spp. Int. J. Parasitol. 43: 647-659.

    Geyer, K.K., Chalmers, I.W., MacKintosh, N., Hirst, J.E., Geoghegan, R., Badets, M., Brophy, P.M., Brehm, K., Hoffmann, K.F. (2013) Cytosine methylation is a conserved epigenetic feature found throughout the phylum Platyhelminthes. BMC Genomics 14: 462.

    Tsai, I.J., Zarowiecki, M., Holroyd, N., Garciarrubio, A., Sanchez-Flores, A., Brooks, K.L., Tracey, A., Bobes, R.J., Fragoso, G., Sciutto, E., Aslett, M., Beasley, H., Cai, X., Camicia, F., Clark, R., Cucher, M., De Silva, N., Day, T.A., Deplazes, P., Estrada, K., Fernández, C., Holland, P.W.H., Hou, J., Hu, S., Huckvale, T., Hung, S.S., Kamenetzky, L., Keane, J.A., Kiss, F., Koziol, U., Lambert, O., Liu, K., Luo, X., Luo, Y., Macchiaroli, N., Nichol, S., Paps, J., Parkinson, J., Pouchkina-Stantcheva, N., Riddiford, N., Rosenzvit, M., Salinas, G., Wasmuth, J.D., Zamanian, M., Zheng, Y., The Taenia solium Genome Consortium, Cai, J., Soberón, X., Olson, P.D., Laclette, J.P., Brehm, K., Berriman, M. (2013) The genomes of four tapeworm species reveal adaptations to parasitism. Nature 496: 57-63.

    Hemer, S., Brehm, K. 2012 In vitro efficacy of the anti-cancer drug Imatinib on Echinococcus multilocularis larvae. Int. J. Antimicrob. Agents, in press.
     
    Nono, J.K., Pletinckx, K., Lutz, M., Brehm, K. 2012 Excretory/secretory products of Echinococcus multilocularis larvae induce apoptosis and tolerogenic properties in dendritic cells in vitro. PLoS Negl. Trop. Dis. 6: e1516.

    Olson, P.D., Zarowiecki, M., Kiss, F., Brehm, K. 2012 Cestode genomics – progress and prospects for advancing basic and applied aspects of flatworm biology. Parasite Immunol., 34: 130-150.
     
    Epping, K., Brehm, K. (2011) Echinococcus multilocularis: molecular characterization of EmSmadE, a novel BR-Smad involved in TGF-b and BMP signaling. Exp. Parasitol. 129: 85-94.

    Förster, S., Günthel, D., Kiss, F., Brehm, K. 2011 Molecular characterization of a serum-responsive, DAF-12-like nuclear hormone receptor of the fox-tapeworm Echinococcus multilocularis. J. Cell. Biochem. 112: 1630-1642.

    Spiliotis, M., Mizukami, C., Oku, Y., Kiss, F., Brehm, K., Gottstein, B. 2010 Echinococcus multilocularis primary cells: improved isolation, small-scale cultivation and RNA interference. Mol. Biochem. Parasitol. 174: 83-87.

    Rassy, D., Bobes, R.J., Rosas, G., Anaya V.H., Hernández, B., Cervantes J., Pedraza S., Morales, J., Villalobos, N., Aluja A., Laclette J.P., Nunes C.M., Biondi G.F., Brehm, K., Fragoso, G., Hernández, M., and Sciutto, E. 2010 Characterization and protective properties of S3Pvac anti-cysticercosis vaccine components: implications for the development of an anti-cestodiasis vaccine. PLoS ONE 5: e11287.

    Brehm, K. 2010 The role of evolutionarily conserved signalling systems in Echinococcus multilocularis development and host parasite interaction. Med. Microbiol. Immunol. 199: 247-259.

    You, H., Zhang, W., Jones, M.K., Gobert, G.N., Mulvenna, J., Rees, G., Spanevello, M., Blair, D., Brehm, K. & McManus, D.P. 2010 Cloning and characterisation of Schistosoma japonicum insulin receptors: potential new intervention targets against schistosomiasis. PLoS ONE 5: e9868.

    Bernthaler, P., Epping, K., Schmitz, G., Deplazes, P. & Brehm, K. 2009 Molecular characterization of EmABP, an apolipoprotein AI binding protein secreted by the Echinococcus multilolcularis metacestode. Infect. Immun. 77: 5564-5571.

    Gelmedin, V., Spiliotis, M. & Brehm, K. 2010 Molecular Characterization of MEK1/2- and MKK3/6-like mitogen-activated protein kinase kinases (MAPKK) from the fox-tapeworm Echinococcus multilocularis. Int. J.Parasitol. 40: 555-567.

    Brehm, K. 2010 Echinococcus multilocularis as an experimental model in stem cell research and molecular host-parasite interaction. Parasitology 137: 537-555.

    Spiliotis, M. & Brehm, K., 2009. Axenic in vitro cultivation of Echinococcus multilocularis metacestode vesicles and the generation of primary cell cultures. Methods Mol. Biol. 470: 245-262.

    Tappe, D., Pukall, R., Schumann, P., Spiliotis, M., Claus, H., Brehm, K. & Vogel, U., 2009. Streptococcus merionis sp. nov., isolated from Mongolian jirds (Meriones unguiculatus). Int. J. Syst. Evol. Microbiol 59: 766-770.

    Gelmedin, V., Caballero-Gamiz, R. & Brehm, K., 2008. Characterization and inhibition of a p38-like mitogen activated protein kinase (MAPK) from Echinococcus multilocularis: antiparasitic activities of p38 MAPK inhibitors. Biochem. Pharmacol. 76: 1068-1081.

    Spiliotis, M., Lechner, S., Tappe, D., Scheller, C., Krohne, G. & Brehm, K., 2008. Transient transfection of Echinococcus multilocularis primary cells and complete in vitro regeneration of metacestode vesicles. Int. J. Parasitol. 38: 1025-1039.

    Zavala-Gongora, R., Derrer, B., Gelmedin, V., Knaus, P. & Brehm, K., 2008. Molecular characterisation of a second structurally unusual AR-Smad without MH1 domain and a Smad4 orthologue from Echinococcus multilocularis. Int. J. Parasitol. 38: 161-176.

    Brehm, K. & Spiliotis, M., 2008. Recent advances in the in vitro cultivation and genetic manipulation of Echinococcus multilocularis metacestodes and germinal cells. Exp. Parasitol. 119: 506-515.

    Brehm, K. & Spiliotis, M., 2008. The influence of host hormones and cytokines on Echinococcus multilocularis signalling and development. Parasite 15: 286-290.

    Tappe, D., Brehm, K., Frosch, M., Blankenburg, A., Schrod, A., Kaup, F.J. & Mätz-Rensing, K., 2007. Echinococcus multilocularis infection of several Old World monkey species in a breeding enclosure. Am. J. Trop. Med. Hyg. 77: 504-506.

    Khayath, N., Vicogne, J., Ahier, A., Ben Younes, A., Konrad, C., Trolet, J., Viscogliosi, E., Brehm, K. & Dissous, C., 2007. Diversification of the insulin receptor family in the helminth parasite Schistosoma mansoni. FEBS J. 274: 659-676.

    Spiliotis, M., Konrad, C., Gelmedin, V., Tappe, D., Brückner, S., Mösch, H.U. & Brehm, K., 2006. Characterisation of EmMPK1, an ERK-like MAP kinase from Echinococcus multilocularis which is activated in response to human epidermal growth factor. Int. J. Parasitol., 36: 1079-1112.

    Zavala-Gongora, R., Kroner, A., Bernthaler, P., Knaus, P. & Brehm, K., 2006. A member of the transforming growth factor-? receptor family of Echinococcus multilocularis is activated by human bone morphogenetic protein 2. Mol. Biochem. Parasitol., 146: 265-271.

    Brehm, K., Spiliotis, M., Zavala-Gongora, R., Konrad, C., Frosch, M., 2006. The molecular mechanisms of larval cestode development: first steps into an unknown world. Parasitol. Int., 55: S15-S21.

    Gelmedin, V., Zavala-Gongora, R., Fernandez, C. & Brehm, K., 2005. Echinococcus multilocularis: cloning and characterization of a member of the SNW/SKIP family of transcriptional coregulators. Exp. Parasitol., 111: 115-120.

    Spiliotis, M., Tappe, D., Brückner, S., Mösch, H.U. & Brehm, K., 2005. Molecular cloning and characterization of Ras- and Raf-homologues from the fox-tapeworm Echinococcus multilocularis. Mol. Biochem. Parasitol., 193: 225-237.

    Aumüller, E., Schramm, G., Gronow, A., Brehm, K., Gibbs, B.F., Doenhoff, M.J. & Haas, H. 2004. Echinococcus multilocularis metacestode extract triggers human basophils to release interleukin-4. Parasite Immunol., 26: 387-395.

    Spiliotis, M. & Brehm, K., 2004. Echinococcus multilocularis: Identification and molecular characterization of a Ral-like small GTP binding protein. Exp. Parasitol., 107: 163-172

    Spiliotis, M., Tappe, D., Sesterhenn, L. & Brehm, K., 2004. Long-term in vitro cultivation of Echinococcus multilocularis metacestodes under axenic conditions. Parasitol. Res., 92: 430-432

    Hubert, K., Zavala-Gongora, R., Frosch, M. & Brehm, K., 2004. Identification and characterization of PDZ-1, a N-ERMAD specific interaction partner of the Echinococcus multilocularis ERM protein Elp. Mol. Biochem. Parasitol., 134: 149-154

    Spiliotis, M., Kroner, A. & Brehm, K., 2003. Identification, characterization and expression of the gene encoding the epidermal growth factor receptor orthologue from the fox-tapeworm Echinococcus multilocularis. Gene, 323: 57-65

    Zavala-Góngora, R., Kroner, A., Wittek, B., Knaus, P. & Brehm, K., 2003. Identification and characterisation of two distinct Smad proteins from the fox-tapeworm Echinococcus multilocularis. Int. J. Parasitol., 33: 1665-1677

    Brehm, K., Wolf, M., Beland, H., Kroner, A. & Frosch, M., 2003. Analysis of differential gene expression in Echinococcus multilocularis larval stages by means of spliced leader differential display. Int. J. Parasitol., 33: 1145-1159

    Konrad, C., Kroner, A., Spiliotis, M., Zavala-Góngora, R. & Brehm, K., 2003. Identification and molecular characterisation of a gene encoding a member of the insulin receptor family in Echinococcus multilocularis. Int. J. Parasitol., 33: 301-312

    Reuter, S., Merkle, M., Brehm, K., Kern, P. & Manfras, B., 2003. The effect of amphotericin B on the larval growth of Echinococcus multilocularis. Antimicrob. Agents Chemother., 47: 620-625

    Scheuring, U.J., Seitz, H.M., Wellmann, A., Hartlapp, J.H., Tappe, D., Brehm, K., Spengler, U., Sauerbruch, T. & Rockstroh, J.K., 2003. Long-term benzimidazole treatment of alveolar echinococcosis with hematogenic subcutaneous and bone dissemination. Med. Microbiol. Immunol., 192: 193-195

    Brehm, K., Hubert, K., Sciutto, E., Garate, T. & Frosch, M., 2002. Characterization of a spliced leader gene and of trans - spliced mRNAs from Taenia solium. Mol. Biochem. Parasitol., 122: 100 – 105

    Brehm, K., Jensen, K. & Frosch, M., 2000. mRNA trans-splicing in the human parasitic cestode Echinococcus multilocularis. J. Biol. Chem., 275: 38311-38318

    Brehm, K., Kronthaler, K., Jura, H. & Frosch, M., 2000. Cloning and characterization of ß-tubulin genes from Echinococcus multilocularis. MMol. Biochem. Parasitol., 107: 297-302

    Brehm, K., Kern, P., Hubert, K., & Frosch, M., 1999. Echinococcosis from every angle. Parasitol. Today, 15: 351-352.

    Brehm, K., Jensen, K., Frosch, P., & Frosch, M., 1999. Characterization of the genomic locus expressing the ERM-like protein of Echinococcus multilocularis. Mol. Biochem. Parasitol., 100: 147-152

    Brehm, K., Ripio, M.-T., Kreft, J., & Vazquez-Boland, J.-A., 1999. The bvr locus of Listeria monocytogenes mediates virulence gene repression by ß-glucosides. J. Bacteriol., 179: 7174-7180

    Ripio, M.-T., Brehm, K.*, Lara, M., Suarez, M., & Vazquez-Boland, J.-A., 1997 Glucose-1-phosphate utilization by Listeria monocytogenes is PrfA dependent and coordinately expressed with virulence factors. J. Bacteriol., 179: 7174-7180

    Brehm, K., Kreft, J., Ripio, M.-T., & Vazquez-Boland, J.-A., 1996. Regulation of virulence gene expression in pathogenic Listeria. Microbiologia SEM 12: 219-236.

    Ripio, M.-T., Dominguez-Bernal, G., Suarez, M., Brehm, K., Berche. P., & Vazquez-Boland, J.-A., 1996. Transcriptional activation of virulence genes in wild-type strains of Listeria monocytogenes in response to a change in the extracellular medium composition. Res. Microbiol., 147: 371-384

    Brehm, K., Haas, A., Goebel, W., & Kreft, J., 1992. A gene encoding a superoxide dismutase of the facultative intracellular bacterium Listeria monocytogenes. Gene, 118: 121-125

    Haas, A., Brehm, K., Kreft, J. & Goebel, W., 1991. Cloning, characterization, and expression in E.coli of a gene encoding Listeria seeligeri catalase, a bacterial enzyme highly homologous to mammalian catalases. J. Bacteriol., 173: 5159-5167



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