Commonly, experiments with human pathogens are conducted at 37°C, as this corresponds to our core body temperature. Yet, Neisseria meningitidis colonizes our nasopharynx, where it is somewhat colder (30-33°C when ambient temperature is 25°C). In recent work conducted at the IHM, Dr. Martin Lappann and colleagues show that the phenotype of meningococci with respect to their ability to colonize the nasopharyngeal niche is significantly enhanced when the bacteria are grown at 32°C instead of 37°C. Upon further elaboration, differential proteome analyses revealed that several proteins, including Bexsero vaccine components NHBA and NMB1030, show a higher relative abundance at 32°C. Furthermore, a link between the temperature-dependent biofilm formation and the extracytoplasmic sigma factor E was drawn.
Impact of moderate temperature changes on Neisseria meningitidis adhesive phenotypes and proteome. Lappann M, Otto A, Brauer M, Becher D, Vogel U, Johswich K. Infect Immun. 2016 Sep 26. pii: IAI.00584-16. [Epub ahead of print]
07.10.2016, 09:40 Uhr